النبذة المختصرة

الاهتمام البحثي بالدراسات الجزيئية من حيث استخلاص المادة الوراثية والترحيل الكهربائي ودراسة التعبير الجيني باستخدام تقنية Real Time PCR Technical experience -Primary and cell line culture, transfection of siRNA, anti-sense oligos, isolation of RNA, DNA and proteins. -Gene microarray analysis, real-time PCR, western blot analysis. -Identification of differential expression of genes using differential display-PCR, sequencing. -Cloning expression, purification and in-gel enzyme assay of recombinant protein. -Molecular characterization of gene-promoters by DNase I foot printing, transient transfection and Luciferase assay of promoter-reporter constructs, and DNA- protein interactions. -Bioinformatics Analysis.