عنوان المقالة:Synthesis of Unnatural Amino Acids for Protein Labeling and Activation: PROTEIN LABELLING
د محمد محمود محمد عبد الفتاح | DR MOHAMMAD MAHMOUD MOHAMMAD ADEL FATAH | 9802
نوع النشر
كتاب
المؤلفون بالعربي
DR MOHAMMAD MAHMOUD
الملخص العربي
Site-specific incorporation of unnatural amino acids into proteins, both in vivo and in vitro, is a promising technology with tremendous potential to advance studies in protein structure and function. The technique allows the incorporation of a vast diversity of functional groups that extends beyond the conventional mutagenesis of the twenty common amino acids. The rapid development of orthogonal PylRS/PylT pairs has resulted in an increasing number of novel unnatural amino acids that can be site-specifically introduced into proteins. This dissertation presents the syntheses of several unnatural amino acids for use in protein labeling and activation. A bipyridine lysine was synthesized for the assembly of metal-binding proteins and its genetic encoding is shown. In addition, unnatural amino acids bearing reactive functionalities for bioconjugation reactions for the selective labeling of proteins were assembled. These include a variety of lysine analogs that can be applied to carbonyl/aminooxy condensations, thiol-ene reactions, and Diels-Alder cycloadditions. Moreover, bioorthogonal reaction partners such as aminooxy dyes, thiol, and tetrazine probes for subsequent protein labeling were prepared. For the photoregulation of protein activity in live cells, several caged tyrosine analogs were synthesized. The assembly of caged phosphoryl tyrosines to study tyrosine
تاريخ النشر
02/05/2016
الناشر
amazon kindle edition
رابط الملف
تحميل (297 مرات التحميل)
الكلمات المفتاحية
Synthesis of Unnatural Amino Acids for Protein Labeling and Activation: PROTEIN LABELLING
رجوع