عنوان المقالة:DETECTION OF RHINOVIRUS AND SOME DNA/RNA VIRUSES BY REVERSE TRANSCRIPTASE REAL-TIME PCR AND THEIR IMMUNOLOGICAL PARAMETERS IN PATIENTS WITH ACUTE RESPIRATORY TRACT INFECTION IN IRAQ: MOLECULAR AND IMMUNOLOGICAL STUDY DETECTION OF RHINOVIRUS AND SOME DNA/RNA VIRUSES BY REVERSE TRANSCRIPTASE REAL-TIME PCR AND THEIR IMMUNOLOGICAL PARAMETERS IN PATIENTS WITH ACUTE RESPIRATORY TRACT INFECTION IN IRAQ: MOLECULAR AND IMMUNOLOGICAL STUDY
الأستاذ الدكتور مشتاق طالب صالح الندا | Dr. Mushtak T. S. Al-Neda | 7353
- نوع النشر
- مجلة علمية
- المؤلفون بالعربي
- المؤلفون بالإنجليزي
- Mushtak T. S. Al-Ouqaili 1, Noor N. Al-Hayani 1 and Israa H. Saadoon 2
- الملخص الانجليزي
- Background and Objectives: Acute upper respiratory tract infections are the most frequent acute illnesses worldwide. This study has been undertaken for determining the diagnostic importance of Human Rhinovirus and other associated DNA/RNA viruses including human Respiratory Syncytial virus; Human Metapneumovirus; Human parainfluenza virus 1-4; human coronavirus; Human B, C, and E Adenovirus; and Human Bocavirus in common cold disease. Further, to detect the role of some immunological parameters including complement three and rhinovirus Ag (RhV-Ag) in rhinovirus infection. Patients and methods: The samples of 84 individuals infected with common cold were collected and studied. Viral nucleic acids either DNA or RNA were extracted and amplified using real-time multiplex PCR. Results: The most common type causative agent was hRv which accounted for 56.1% followed by hRv+hRSV-hMpv, 12.19% and hRv+hAdv-hBov, 9.75% respectively. Also, the most rarely infection was hRSV-hMpv which found only in one case. On the other hand, complement three was used as a potent immuno-parameter to detect the level in a sample of 80 tested individuals infected with human rhinovirus at which it was found that there is a simple linear correlation coefficient matrix of the variables C3 and RhV-Ag. Conclusion: The study suggested that multiplex RT-PCR is a rapid, cost-effective, specific and highly sensitive method for detection of respiratory viruses including rhinovirus. Further, this study has focused a highlight on the critical roles of C3 in the pathogenesis of rhinovirus infection. Furthermore, RhV-Ag was found to be significantly correlated with the complement C3 at p<0.05 and RhV-Ag cannot be predicted using complement C3.
- تاريخ النشر
- 01/05/2019
- الناشر
- INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH
- رقم المجلد
- 10
- رقم العدد
- 5
- ISSN/ISBN
- 0975-8232 / 2320-5148
- رابط DOI
- 10.13040/IJPSR.0975-8232.10(5).1000-11
- رابط الملف
- تحميل (151 مرات التحميل)
- رابط خارجي
- https://ijpsr.com/bft-article/detection-of-rhinovirus-and-some-dna-rna-viruses-by-reverse-transcriptase-real-time-pcr-and-their-immunological-parameters-in-patients-with-acute-respiratory-tract-infection-in-iraq-molecular-and-imm/?view=fulltext
- الكلمات المفتاحية
- Real-Time PCR, Human rhinovirus, Common Cold