عنوان المقالة: Increase production of Prodigiosin pigment from mutant Serratia marscences against bacterial malignant tumors (Cancers) by physical mutagenesis
Nebras Rada Mohammed1); Hanaa Salih Sabaa2) and Taif Hussain3
الملخص الانجليزي
The goal of study in order that increase expression of prodigiosin pigment from mutant Serratia marscences with study the effect antimicrobial activity of prodigosin pigment against bacterial isolated from malignant tumors patients. The isolates collected 125 isolates from Baghdad hospitals linked with Leukemia, Belly cancer, Cochlea cancer, Spleen cancer, Renal cancer and Liver cancer.
Outcome of isolation were 20 isolates (17%) Staphylococcus aureus; 18 (14%) Escherichia coli ; 9(7%) Acinetobacter baumanii; 9 (7%) Salmonella spp.; 9 (7%) Streptococcus sp .; 5 (4%) of Klebsiella pneumonia; 5 (4%) of Pseudomonas aeruginosa,; 5(4%) Pantoea spp.; 5(4%) Aeromonas spp.; 5(4%) Morganella morganii ; 5 (4%) Staphylococcus epidermidis and 5(4%) Micrococcus sp. , also collecting Serratia marscences 25(20%) from total 125 isolates from different infections of patients.
The physical mutagenesis achieved by Nd:YAG lasers in 500 pulse, the results of mutagenesis exhibit high expression of prodigosin after physical mutagenesis to isolates of S.marscences which was 15 isolates (60%) and compared prodigiosin production before the mutagenesis which was 25 isolates (total isolates of S.marscences);the bacteria were also exposed to Alpha, Gamma and Beta rays to
2
different radiosources (isotopes) including Am241(1Mci) emitte Gamma and Alpha ray with dose 0.31993*10-4KGy for Gamma ray and 1.4157 KGy at 3 hr. for Alpha
particles.; Sr90 (9Mci) emitte Beta ray in dose 1.973*10-8 KGy at 3 hr.; Cs137 (1Mci) emitte Beta and Gamma ray in dose 1.3158*10-8 KGy at 2 hr. for Beta ray and 1.973*10-10 KGy at 3 hr. for Gamma ray; Na22(1Mci) emitte Beta and Gamma ray in dose 1.533*10-8 KGy at 3hr..The results of exposure showed high improvement production of prodigosin in Americium were 21 isolates (84%), Strontium 17(68%), Cesium 23(92%) and Sodium 12(48%) that give high expression of prodigosin pigment.
The prodigiosin dye was taken away of S.marscences via Chloroform in acid-base medium method and purification by TLC (Thin Layer Chromotography mechanization) by employ Silica gel sheet.
The antibacterial effect of antibiotics done to S.marscences before the physical mutagenesis and after physical mutagenesis into 28 antibiotics in order that determine susceptibility by resistance and sensitive, results the study of resistance of S.marscences before physical mutagenesis improved turn off into sensitive of different antibiotics were E.coli turn off sensitive to ciprofloxacin after physical mutagenesis, as well A.baumanii turn off sensitive to Amikacin, Piperacillin; Morganella morganai turn off sensitive to Pipracillin; K.pneumonia turn off sensitive to Ciprofloxacin, Pantoea spp. turn off sensitive Tetracyclin; S.aureus turn off sensitive to Tetracyclin ; S.epidermidis turn off sensitive Methicillin and Streptococcus turn off sensitive Penicillin G.
نبراس رضا محمد | Nebras Rada Mohammed | 18743