Shayma Thyab Gddoa Al‐sahlany, Ammar B. Altemimi, Alaa Jabbar Abd Al‐Manhel , Alaa Kareem Niamah , Naoufal Lakhssassi and Salam A. Ibrahim
الملخص العربي
variety of organisms produce bioactive peptides that express inhibition activity against
other organisms. Saccharomyces cerevisiae is considered the best example of a unicellular organism
that is useful for studying peptide production. In this study, an antibacterial peptide was produced
and isolated from Saccharomyces cerevisiae (Baker’s yeast) by an ultrafiltration process (two
membranes with cut‐offs of 2 and 10 kDa) and purified using the ÄKTA Pure 25 system.
Antibacterial peptide activity was characterized and examined against four bacterial strains
including Gram‐positive and Gram‐negative bacteria. The optimum condition for yeast growth
and antibacterial peptide production against both Escherichia. coli and Klebsiella aerogenes was 25–30
°C within a 48 h period. The isolated peptide had a molecular weight of 9770 Da, was thermostable
at 50–90 °C for 30 min, and tolerated a pH range of 5–7 at 4 °C and 25 °C during the first 24 h,
making this isolated antibacterial peptides suitable for use in sterilization and thermal processes,
which are very important aspect in food production. The isolated antibacterial peptide caused a
rapid and steady decline in the number of viable cells from 2 to 2.3 log units of gram‐negative
strains and from 1.5 to 1.8 log units of gram‐positive strains during 24 h of incubation. The isolated
antibacterial peptide from Saccharomyces cerevisiae may present a potential biopreservative
compound in the food industry exhibiting inhibition activity against gram‐negative and
gram‐positive bacteria.
تاريخ النشر
11/03/2020
الناشر
Foods
رقم المجلد
9
رقم العدد
رابط DOI
doi:10.3390/foods9030324
الكلمات المفتاحية
Saccharomyces cerevisiae; bioactive peptide; antibacterial; ÄKTA pure
علاء كريم نعيمة | Alaa Kareem Niamah | 5212