عنوان المقالة:تواجد جينات الESBL في بكتريا ال Escherichia coli وcinetobacter baumannii المعزوله من مختلف العينات السرسريه Existence ofaESBL genes in Escherichia coli and Acinetobacter baumannii isolated from different clinical specimens
Huda H.Al-Hasnawy, Rasha Hadi Saleh, CBara ́ Hamid Hadi
الملخص الانجليزي
Background: The antimicrobialaresistance are a worldwide increasing and importantaproblem in health care field . ESBLs represent a major group of β-lactamases enzymes thatamostly produced by gramanegative bacteria, and confer resistance toaβ- lactam antibiotics, so the detectionaof these enzymes are very importantafor optimal patients care. Objective: The aim ofathis study was toadetermine the antibiotic profile forathe isolates with theaprevalence of ESBLaproducingaE. coli and A.baumanniiaisolatesawhich recovered from clinicalaspecimens by phenotypic andagenotypic methods.Material and Methods: A total ofa100 clinicalasamples (urine,asputum and swabs ofawound,aburn and ear) were included in thisastudy. Allbacterial isolates were subjected to the cultural,amicroscopical, and biochemical examinationsamethods, confirmed byaVITEK-2 system.Antibiotic sensitivity was performedaby using disk diffusionamethods. Investigation of β-lactamaseaand extended spectrum β-lactamasea(ESBL) production for both isolates wasaperformed using rapid standard iodometricaassay and double discasynergy method(DDST). PCR technique was conducted ato detect β-lactamase genes,aTEM-2 and SHV and theagene OXA-51 likeawas used toaconfirm A.baumannii isolates. Results: The results showed thataout of 100 clinical samples, 15 isolates belonged toa E.coli and 13 isolates belonged toa A. baumannii.E.coliaand A.baumannii isolates showed aresistance to ß-lactam antibiotics and vast amajority of isolatesawere resistant to a minimum ofathree classesaof antibiotics, ahence the isolates were considered to beamultidrug resistant. Phenotypically, the results revealedathat (100%)and (15.3%) of E. coli and A.baumannii isolates producedaβ-lactemases while Extended spectrum β-lactemases (ESBLs)aproduction found in(100%) and (23%) of E. coli and A.baumannii isolaterespectively. PCR technique was performed to detect ESBLs genes bla -TEM2 andbla-SHV, the results revealed that (86.6%) and ( 6.6%) of E.coli isolates carried bla-TEM2, and bla-SHV, while (15.3%) and (7.6%) ofA.baumannii isolates carried abla-SHV andabla-TEM-2genes respectively.Conclusion: There are a high resistance of bacterial isolates to most antibiotic especially to β –lactam antibiotics. ESBL genes distributed inclinical isolates of E. coli
(PDF) Existence ofaESBL genes in Escherichia coli and Acinetobacter baumannii isolated from different clinical specimens. Available from: https://www.researchgate.net/publication/338541025_Existence_ofaESBL_genes_in_Escherichia_coli_and_Acinetobacter_baumannii_isolated_from_different_clinical_specimens [accessed Oct 26 2020].
رشا هادي صالح | Rasha hadi saleh | 5388