Fatima.R.Abdul*, Nehad.A.Taher*,1,Ashraf S.Hassan* and Enaam H. Batah
الملخص العربي
From a large number of bacterial samples collected from different hospital in Iraq in central
health laboratory ,only ten isolates were identified primary as Vibrio. A number of morphology and
biochemical test were carried out to complete this identification that showed all bacterial isolates were
related to Vibrio cholerae .In this study all Vibrio isolates were investigated for Bio typing and the
result showed that all (10) isolate were related to (Eltor biotypes) .Also, the susceptibility test towards
eight antibiotics were carried out .
Results shows that ciprofloxacin , Norfloxacin, Erythromycin, Ampicillin, ceftriaxone and
Amikacin were the most effective antibiotics and their resistance percentage were 20%,20%,20,
20,30% and 30% respectively ,While Chloramphenicol and Co- trimoxazole were less effective and
their resistance percentage were 90% both of them. Three (3,5,6) isolates V. cholerae were selected
depending on results of antibiotics sensitivity tests as showed multiple –antibiotics
resistance(100%).
Then tested to study the effect of coumarin derivatives compounds (1, 2, 3 ) which showed
inhibitory effect on V. cholera (3,5,6) isolates and the compound ( 3 ) showed the highest antibacterial
activity of (12,15,14 mm) of inhibition zone diameter against V. cholera (3,5,6) isolates respectively.
Also, these Iraqi isolates (3,5,6) used to test the effect of acridine orange (0.1%) as acuring
agent , the results showed that all (3) isolates V. cholerae were sensitive to (ciprofloxacin, ceftrixone
and Norfloxacin), While the rest were resistance to remained five antibiotics.
The results of Agarose –gel electrophoresis of both normal V. cholerae (3,5,6) and cured
isolates showed the presence of chromosomal and plasmid DNA bands in the normal case ,While only
chromosomal DNA bands occur with V. cholerae (isolate 8) treated with an acridine orange at
concentration of (10-2 to 10-4).
أ.م. اشرف سامي حسن | Assist prof.Ashraf S.Hassan | 7149