د هند حسين عبيد و حسن عبود كريم و د. أحمد أسمر منخي
المؤلفون بالإنجليزي
Hind Hussein Obaid , Hassan Abood Kareem , Ahmed Asmer Manghe
الملخص العربي
لاتوجد خلاصة باللغة العربية
الملخص الانجليزي
Smear-negative tuberculosis (TB) is a common problem in diagnosis by Zehil-Neelsen (ZN). The comparison between the classical methods (ZN test, culture on Lownestien Jenison (LJ) and Middle Brook 7H9 media) and Immunological methods (Mycobacterium Growth Identification Test (MGIT) and Rapid Chromatography Immunoassay test) used in the present study and to assessment of tests that show rapidly and accuracy in diagnostic TB. The prospective study done in Ministry of Health / Specialized Center of Chest Respiratory Disease in Baghdad. A total of 110 specimens collected in this center, that include 85 specimens for patients and 25 for control healthy. Specimens collection included sputum and blood .Tuberculosis patients were divided into four subgroups depending on result of ZN test and history of disease. Classical methods showed positive results following ZN 50(58.82%), LJ medium 81(95%) and Middle-Brook 7H9 medium 80(94%). Immunological methods showed positive results following: rapid test 40(47%) and MGIT 81(100%). 35 specimens from all specimens (85) represent for smear negative patients, that showed positive results in LJ media (100%), Middle Brook 7H9 media (94.2%) ; MGIT (100%) and rapid test (48.57%).Methods showed at culture on LJ medium better from ZN test and Middle brook 7H9 medium, high positive results for culture on LJ medium other than low in ZN test. Middle Brook 7H9 medium is rapid and easy to use but requirement of sub culture on solid media for mycobacterial identification and drug sensitivity. Finally detection of MTB by using LJ medium is time consuming, but it still the gold standard for mycobacterial detection. To compare the detection result of immunological methods with classical methods in our study. HG
تاريخ النشر
23/03/2016
الناشر
المؤتمر العلمي الدولي الثالث للتخصصات الطبية والصحية/ الجامعة التقنية الوسطى
هند حسين عبيد | Hind Hussein Obaid | 27092