عنوان المقالة: DETERMINATION OF THE OPTIMUM CONDITIONS FOR BIOSURFACTANT PRODUCTION BY LOCAL ISOLATE OF LACTOBACILLUS PLANTARUM AND EVALUATE ITS ANTIMICROBIAL
Eighty five local isolates of Lactobacillus sp. which were isolated from different sources and
identified by biochemical test then subjected to the primary and secondary screening
processes to select the active Lactobacillus sp. isolate for biosurfactant production. Among the
isolates screened, twenty six isolates with maximum for tests in primary screening were
selected for secondary screening. It has been found that Lactobacillus sp. ADK2 had the
highest productivity of the biosurfactant. The selected isolate with highest level of
biosurfactant activity was identified as Lactobacillus plantarum ADK2 according to PCR
technique. The optimum conditions of biosurfactant production by isolate Lactobacillus
plantarum ADK2 using submerged fermentation were obtained in the synthetic mineral salt
medium (MSM) and natural BCDFTM medium the best production medium separately, 1.5%
(lactose and egg) as the best carbon source, 2% meat extract and 3.5% Pease as nitrogen
source, temperature 30 °C for two media and pH 5 with pH 3 in MSM and BCDFTM
respectively, after 96 hr and 72 hr in MSM and BCDFTM respectively of incubation period
د. علياء عبد الحسين جميل | Dr. Aleaa Abdulhussein Jameel | 2013