عنوان المقالة: Bacillus sp. R2 Agarase Production: Medium Composition and Fermentation Conditions Optimization
بن عمر شبة | benamar cheba | 15789
نوع النشر
مقال علمي
المؤلفون بالعربي
بن عمر شبة
المؤلفون بالإنجليزي
Ben Amar Cheba
الملخص الانجليزي
Abstract: Agar, which can be degraded by agarase, is composed of agarose and agaropectin. Agarases producing microorganisms have been isolated from many sources, including seawater, marine sediments, marine algae, marine mollusks, fresh water, and soil. The present study was firstly planned to investigate the effect of medium composition and culture conditions on agarase production by Bacillus sp. R2 in shake flasks (submerged fermentation), using one factor at time technique (OFAT). The highest agarase activity was obtained in 0.2% agar as essential carbon source, 0.2% lactose as secondary carbon source, 0.5% NH4Cl as nitrogen source, 5% NaCl and 5% culture seed as best inoculum size. The production also was optimum at initial pH: 7, temperature 30 °C, and 24 h incubation period at 150 rpm shaking. The agarase hyperactivity in this cost-effective economic medium composition and culture condition enlighten the potential application of Bacillus sp. R2 as promising new source for agarase production. Keywords: Bacillus sp. R2; agarase; submerged fermentation; production; optimization
تاريخ النشر
07/08/2022
الناشر
WASSN 2021, 20
رقم المجلد
رقم العدد
20
ISSN/ISBN
ISSN: 2766-7715
الصفحات
6
الكلمات المفتاحية
Keywords: Bacillus sp. R2; agarase; submerged fermentation; production; optimization
رجوع