In this study, new azetidine [A1-A5] compounds were prepared by the reaction of schiff base compounds with chloroacetyl chloride in 1,4-dioxane. The prepared compounds were characterized by physical properties, UV-Vis, FT-IR and 1H-NMR spectral and C.H.N analysis. TLC checked the purity for these compounds. The antibacterial activities were studied against different kinds of bacteria, namely Eschershia coli and Klebislla Pneumonia Gram (-) ve, Staphylococcus aureus and Staphylococcus epidermidis Gram (+) ve. In addition, evaluation of laser efficacy is showed for the compounds (A1-A5) were radiated by laser for (10, 20, 30) seconds. It was observed that the compounds were not affected or polymerize when the melting point and color were measured.