عنوان المقالة:تاسيس وتوصيف خط سرطان الغدة اللبنية الفاري القابل للغرس في الفئران Establishment and characterization of AN3 first murine mammary adenocarcinoma transplantable tumor line in Iraq
Ahmed Majeed Al-Shammari, Nahi Yaseen and Mohammed Alwan
الملخص العربي
تم زراعة عينة سرطان الغدة اللبنية الفاري الناشيء في فارة كبيرة العمر بشكل تلقائي في فئران مضعفه مناعيا ومن ثم نما الورم فيها وتم اخذ عيناات منه وتم تنميتها في فاران ذات مناعه طبيعيا واستمرت هذا العملية عدة سنوات وتمت دراسة مواصفات هذا الخط السرطاني في هذا العمل البحثي.
الملخص الانجليزي
Spontaneous murine mammary adenocarcinoma of aged female mouse was allotransplanted into immunosuppressed mice and successfully adapted for growing in immunocompetant mice for more than 50 passages in vivo. Take rate, tumor doubling time and growth rate were evaluated, cross examination showed no invasion or infiltration and highly angiogenesis nature of the tumor with milky like secretion. Histopathological examination performed at each passage showed mammary adenocarcinoma picture which characterized with presence of glandular structures, special stain as Mallory triple stain and PAS stain was performed to study the general tumor structure. Late passages (after passage 25) showed highly metastatic nature especially in to the liver. Ultrastructural study proved the secretory nature of that tumor by presence of secretory vacuoles. Chromosomal analysis showed polyploidy chromosomes in the most cells. AN3 transplantable tumor line may be used as animal tumor model in the development and testing of new anticancer agents (chemotherapy, radiotherapy and biological therapy), and it is useful in tumor pathology and biology studies.
أحمد مجيد حمزة الشمري | Ahmed Majeed Al-Shammari | 6399