عنوان المقالة: Detection of Substitution Mutations of Malassezia furfur isolated from Iraqi Immunocompetent and Immunocompromised Patients with Seborrheic Dermatitis
Seborrheic dermatitis (SD) is a chronic widespread
dermatological disorder, it is characterized with
Scaling, redness and inflammation of infected site.
Immunocompromised patients have immune systems
deficient such as AISD, diabetes mellitus, organ
recipients and chemotherapy. The causative agent of
SD is unknown, but it has been related to the
opportunistic yeast of Malassezia furfur isolated from
both healthy skin and at sites affected with SD.
Sequencing of LSU region represents the most useful
techniques in Malassezia furfur identification. Eighty
patients suffering from Seborrheic dermatitis disease
were selected from two groups: (i) Those with
seborrheic dermatitis and (ii) Immunocompromised
patients with SD. The age of studied population was
ranging from 1 to 60 years old.
Direct examination of specimens was performed using
KOH (10%) and staining with lacto phenol cotton blue
and indirect examination using culture media and
molecular methods to differentiate for M. furfur from
other species.
According to the gender, males were more infected with
SD than female. According to age groups, the most
frequent group was 41-60 years old. The percentage of
positive results of M. furfur among immunocompetent
and immunocompromised seborrheic dermatitis
patients was 15% and 12.5% respectively. Upon
sequencing of twelve isolates of M. furfur which sent
for sequencing, 2 out of 12 isolates appeared as
substitution mutations, the origin of these isolates are
immunocompromised patients with SD disease. The
substitution mutations may play an important role in
the pathogenesis of immunocompromised with SD
disease. In addition, molecular methods were more
rapid and specific in comparison with cultural methods
to identify M. furfur.
تاريخ النشر
03/02/2019
الناشر
Research Journal of Biotechnology
رقم المجلد
14
رقم العدد
الصفحات
1-5
الكلمات المفتاحية
Immunocompromised patients and Molecular methods.Keywords: Malassezia furfur, Seborrheic dermatitis,
محمد جبير مهيدي | Mohammed Jobair Muhaidi | 3585