عنوان المقالة: Genetic Polymorphism in ND1 Gene of Hydatid Cyst in Iraqi goats
محمد جبير مهيدي | Mohammed Jobair Muhaidi | 3585
- نوع النشر
- مجلة علمية
- المؤلفون بالعربي
- د.محمد جبير مهيدي
- المؤلفون بالإنجليزي
- Mohammed Jobair Muhaidi
- الملخص الانجليزي
- Hydatid cysts were taken from 18 goats living in different places in Iraq after diagnosing and specifying the place of the injury for the period from July 2016 till February 2018. DNA was extracted from (protoscoleces) by using the mechanical crashing method to gain an enough quantity of the DNA to perform the partial diagnostic. NADH dehydrogenase subunit 1 Gene (ND1) was used as a goal after designing the specific primers for study. Results showed that a DNA fragment was obtained from all the samples that have been studied where the primary detection of the gene depends on the molecular size of about 800 bp after electrophoresis on Agaros gel. After that, the results were refined by using Geneaid Kit, Kuria and the purity of the DNA was measured by using Spectrophotometer by measuring the wave lengths 260 and 280 and then specifying the value of (OD). After receiving the gene ND1 series, they were matched with International Gene Bank NCBI where all the symbols gave matching results with G1 sheep strain.
- تاريخ النشر
- 03/02/2019
- الناشر
- Research Journal of Biotechnology
- رقم المجلد
- 14
- رقم العدد
- الصفحات
- 19-22
- رابط الملف
- تحميل (59 مرات التحميل)
- الكلمات المفتاحية
- Sequences.Keywords: Hydatid cyst in goat, G1 strain, ND1 gene, Goat,