عنوان المقالة:Molecular detection of Capillaria philippinensis: an emerging zoonosis in Egypt
أيمن البدرى | Ayman A. El-Badry | 6961
نوع النشر
مجلة علمية
المؤلفون بالعربي
El-Dib, N.A., El-Badry, A.A., Ta-Tang, T.H., Rubio, J.M.
الملخص العربي
Human infection with Capillaria philippinensis is accidental; however, it may end fatally if not diagnosed and treated in the proper time. The first case was detected in the Philippines in 1963, but later reported in other countries around the world, including Egypt. In this report, molecular diagnosis using a specific nested PCR for detection of C. philippinensis in faeces is described based on the amplification of small ribosomal subunit. The test showed sensitivity and specificity, as it detected all the positive cases and gave no cross-reaction with human DNA and DNA of other tested parasites. This method can be very useful not only for improvement of diagnosis, but also to understand the different environmental routes of transmission by detection of C. philippinensis DNA-stages in the possible fish intermediate hosts and reservoir animal host, helping to improve strategies for surveillance and prevention of human disease.
تاريخ النشر
07/01/2015
الناشر
Exp Parasitol.
رابط DOI
10.1016/j
رابط خارجي
http://www.sciencedirect.com/science/article/pii/S0014489415001009
الكلمات المفتاحية
Capillaria philippinensis; Nested PCR; Copro-DNA; Mal-absorption; Capillariasis
رجوع