عنوان المقالة:Effect of pyocyanin as secondary metabolite on pseudomonal biofilm and in increasing the resistance degree to antipseudomonal agents Effect of pyocyanin as secondary metabolite on pseudomonal biofilm and in increasing the resistance degree to antipseudomonal agents
الأستاذ الدكتور مشتاق طالب صالح الندا | Dr. Mushtak T. S. Al-Neda | 8248
نوع النشر
أخرى
المؤلفون بالعربي
المؤلفون بالإنجليزي
Al-Moghira Khairi Al-Qaysi1 , Mushtak T. S. Al-Ouqaili2 *, Safaa Abed Latef Al-Meani3
الملخص الانجليزي
Background: Pseudomonas aeruginosa produced the maximum amount of pyocyanin pigment as a secondary metabolite as a virulence factor that responsible for increase pathogenesis. The study aims to improve our understanding of the emergence of pseudomonal biofilm formation by pyocyanin production. The correlation between minimal inhibitory concentration (MICs) values of ceftazidime, piperacillin, and ciprofloxacin with pyocyanin production is also targeting in this study. Materials and Methods: A total of 96 isolates from patients with catheter-associated urinary tract and wound infections were included in this study. Pyocyanin production was determined. The antimicrobial susceptibility tests including, Kirby– Bauer and E-test, were estimated. Finally, the detection of biofilm production, which includes microtiter plate assay and biofilm formation on FBCL, was done. Results: One hundred thirty-eight isolates of P. aeruginosa, isolates that produced biofilm were 96 (69.5%). There is a progressive relationship between pyocyanin production level and biofilm production. Furthermore, there is a relationship between MICs values of ceftazidime and pyocyanin production level. Conclusion: Our suggestion showed that pyocyanin could be one of the factors that help to induce biofilm formation. Wherefore, sessile cells were showed an increase in isolates that have higher pyocyanin levels more than others. Furthermore, pyocyanin increases the resistance against ceftazidime, which could be of clinical significance.
تاريخ النشر
25/03/2020
الناشر
Drug Invention Today
رقم المجلد
14
رقم العدد
3
ISSN/ISBN
0975-7619
رابط الملف
تحميل (151 مرات التحميل)
رابط خارجي
https://jprsolutions.info/files/final-file-5f7ab2270d7215.31138164.pdf
الكلمات المفتاحية
Minimal inhibitory concentration, Pseudomonal biofilm, Pyocyanin
رجوع