عنوان المقالة:التوصيف النسيجي المناعي الكيميائي لتجمعات الخلايا العصبية كاندي (كيسبيبتين/نيوروكينن ب/داينوروفين) وتجمعات خلايا الكيسبيبتين العصبية في المنطقة القبل بصرية وذلك في منطقة تحت المهاد خلال دورة الشبق (الشياع) في عجلات الأبقار Immunohistochemical characterization of the arcuate kisspeptin/neurokinin B/dynorphin (KNDy) and preoptic kisspeptin neuronal populations in the hypothalamus during the estrous cycle in heifers
أحمد سعد أحمد حسانين | Ahmed Saad Ahmed Hassaneen | 7536
نوع النشر
مجلة علمية
المؤلفون بالعربي
أحمد سعد أحمد حسانين - يوسوكا نانيوا - يوتا سوتومي - شوتشي ماتسوياما - كوجي كيمورا - ناهوكو ايدا - ناوكو اينوي - يوشيهيسا أوينوياما - هيروكو تسوكامورا - كيه-ايتشيرو مايدا - فوكو ماتسودا - ساتوشي أوكرا
المؤلفون بالإنجليزي
Hassaneen, Ahmed Saad Ahmed; Naniwa, Yousuke; Suetomi, Yuta; Matsuyama, Shuichi; Kimura, Koji; Ieda, Nahoko; Inoue, Naoko; Uenoyama, Yoshihisa; Tsukamura, Hiroko; Maeda, Kei-ichiro; Matsuda, Fuko; Ohkura, Satoshi
الملخص الانجليزي
Elucidating the physiological mechanisms that control reproduction is an obvious strategy for improving the fertility of cattle and developing new agents to control reproductive functions. The present study aimed to identify kisspeptin neurons in the bovine hypothalamus, clarifying that a central mechanism is also present in the cattle brain, as kisspeptin is known to play an important role in the stimulation of gonadotropin-releasing hormone (GnRH)/gonadotropin secretion in other mammals. To characterize kisspeptin neurons in the bovine hypothalamus, the co-localizations of kisspeptin and neurokinin B (NKB) or kisspeptin and dynorphin A (Dyn) were examined. Hypothalamic tissue was collected from Japanese Black or Japanese Black x Holstein crossbred cows during the follicular and luteal phases. Brain sections, including the arcuate nucleus (ARC) and the preoptic area (POA), were dual immunostained with kisspeptin and either NKB or Dyn. In the ARC, both NKB and Dyn were co-localized in kisspeptin neurons during both the follicular and luteal phases, demonstrating the presence of kisspeptin/NKB/Dyn-containing neurons, referred to as KNDy neurons, in cows. In the POA, no co-localization of kisspeptin with either NKB or Dyn was detected. Kisspeptin expression in the follicular phase was higher than that in the luteal phase, suggesting that kisspeptin expression in the POA is positively controlled by estrogen in cows. The kisspeptin neuronal populations in the ARC and POA likely play important roles in regulating the GnRH pulse and surge, respectively, in cows.
تاريخ النشر
01/05/2016
الناشر
Journal of Reproduction and Development
رقم المجلد
62
رقم العدد
5
ISSN/ISBN
Online ISSN : 1348-4400
رابط DOI
https://doi.org/10.1262/jrd.2016-075
الصفحات
471-477
رابط الملف
تحميل (0 مرات التحميل)
رابط خارجي
https://www.jstage.jst.go.jp/article/jrd/62/5/62_2016-075/_pdf/-char/en
الكلمات المفتاحية
Estrogen, Estrous cycle, Heifers, Immunohistochemistry, KNDy
رجوع