عنوان المقالة:Protective Effect of Melittin against Gastric Inflammation in Mice
عبير الأحمري | Abeer Alahmari | 5448
- نوع النشر
- مجلة علمية
- المؤلفون بالعربي
- Tarek Rahmy, Abeer Alahmari, Faiza Abdu and Osama Abu-Zinadah
- الملخص العربي
- The major compound of bee venom, melittin, has been used as an anti-inflammatory reagent for decades. However, the potential of melittin to ameliorate stomach inflammation is unknown. Our aim was to investigate the effect of melittin on indomethacin-induced gastrointestinal inflammation. Adult male Albino mice (Swiss mice strain) were randomly divided into four groups (7 mice each group): control group; indomethacin-treated group (50 mg/kg) for 1day; melittin treated group (10 or 40 μg/kg) for 3,5 or 10 days; and melittin/indomethacin treated group. The results of the histological studies showed that the effect of indomethacin on the stomach tissue of mice included superficial erosion and exfoliation of some epithelial cells to the gastric lumen, also large areas full of numerous inflammatory cells were seen at the submucosa and extend to different parts of the lamina properia. Besides the depletion of antibody of epithelial membrane antigen (EMA) in the stomach tissue. In accordance with the results of in vivo experiments, melittin doses (10 and 40 μg/kg) inhibited histological and immuonohistochemical changes in the stomach during inflammation induced by indomethacin, where the gastric tissues showed more or less intact mucosal epithelial cells and the submucosal inflammatory cells were less in number compared to those recorded in the gastric tissues of indomethacin-treated mice. Also normal histological structures of the gastric glands, the muscularis mucosa, the muscularisexterna and the serosa were recorded. On other hand, it was showed the reactivity of EMA in the stomach tissues were reduced under the effect of indomethacin treatment, while melittin restored the reduction in EMA reactivity induced by indomethacin in tissues, this observed could be attributed to the protective effect of melittin against the abnormality cases of epithelial cells. In conclusion, these results clearly indicate that melittin provided protection against indomethacin-induced gastrointestinal inflammation through its ability to protect the epithelial lining cells of the stomach by suppressing the activity of phospholipase and protease enzymes which may contribute to the exfoliation and erosion of the mucosal epithelial cell.
- تاريخ النشر
- 16/06/2013
- الناشر
- Life Science Journal
- رابط الملف
- تحميل (190 مرات التحميل)
- رابط خارجي
- http://www.lifesciencesite.com.