This study aimed to detect of methicillin resistant S. aureus (MRSA) which caused neonatal infections and to
detect the genes being responsible of antibiotic resistance in these bacteria by molecular technique (PCR). Antibiotic
sensitivity test results demonstrated that, S. aureus isolates were highly resistant against most antibiotics, 100% for each
AMP, AMC, CTX, CTR, CAZ, KF and K. whereas it's resistance ≥70% for most other antibiotics. While 0% resistance
(100% sensitivity) was shown in each IPM, MRP. The results of minimum inhibitory concentration MIC by using HiComb
test showed that S. aureus has 100% resistance for both MET and OX with MIC values ranged between (5-240µg/ml and
32-256µg/ml respectively). While 0% resistance reported for VA (0.024-0.512µg/ml). PCR results demonstrated that,
among 35 isolates of S. aurus were determined previously as MRSA by phenotypic methods, 91.4% have mecA gene, 37%
have nuc gene and 2.9% have vanA
تاريخ النشر
05/10/2014
الناشر
International Journal of Medicine and Pharmaceutical Sciences (IJMPS)