عنوان المقالة:Molecular characterization of Cutaneous Leishmaniasis in Al-Madinah Al-Munawarah province, western Saudi Arabia
أيمن البدرى | Ayman A. El-Badry | 6970
نوع النشر
مجلة علمية
المؤلفون بالعربي
El-Beshbishy, H.A., Al-Ali, Kh.H., El-Badry, A.A.
الملخص العربي
BACKGROUND: Leishmaniasis is a parasitic disease affecting a large number of people worldwide. In this study we carried out the molecular characterization of cutaneous leishmaniasis (CL) in Al-Madinah Al-Munawarah Province, Saudi Arabia, confirming Leishmania major and Leishmania tropica as the prevalent species using molecular techniques. METHODS: One hundred and five patients with suspected CL were identified from four different localities in Al-Madinah Al-Munawarah Province and Al-Miqat Hospital, Al-Madinah, Saudi Arabia. Thirty-four of the 105 patients were selected at random for molecular investigation. RESULTS: Characterization of CL species by internal transcribed spacer 1 (ITS1) PCR-restriction fragment length polymorphism (RFLP) and kinetoplast DNA (kDNA) PCR established L. major and L. tropica as the causative organisms. kDNA PCR had a sensitivity of 90.7%, whereas ITS1 PCR had a sensitivity of 70.1%, thus facilitating the diagnosis and species identification. Parasite culture alone detected 39.2% and smear alone 55.3% of the positive samples. With the exception of kDNA PCR, all other assays were 100% specific. CONCLUSIONS: This study provides the first findings for the comprehensive molecular characterization of CL in Saudi Arabia.
تاريخ النشر
05/01/2013
الناشر
Int J Infect Dis.
رابط DOI
10.1016/j.
رابط خارجي
http://www.ijidonline.com/article/S1201-9712(12)01307-0/pdf
الكلمات المفتاحية
Cutaneous leishmaniasis PCR-RFLP ITS1 PCR Patients Al-Munawarah Saudi Arabia
رجوع