Mycotoxins are fungal toxic metabolites which naturally contaminate food and feed. Aflatoxins, when ingested, inhaled or adsorbed through the skin, have carcinogenic, hepatotoxic, teratogenic and mutagenic effects in human and animals. Genetically, Aspergillus was one of the best studied fungi and the complete genome sequence of A. flavus in addition to several strains of A. flavus group strains are available now at the National Center for Biotechnology Information (NCBI) (Rodrigues et al., 2007). Phylogenetic analysis of ITS region seems to be a useful tool to provide taxonomical information about ecological genotypes. In this study, the results showed the DNA chain reaction using primers ITS1-ITS4 located within the ribosomal gene 18S rRNA and a clear band resulting from the process of doubled. It has been used BLAST tool to find parallelism and compared nucleotide sequence for ITS region in ribosomal gene S18 with nucleotide sequence data base the other strain in the National Centre for Biotechnology Information NCBI and Gene Bank results showed 98% ratio match for isolat
د حليمة زغير حسين | Halima Zugher Hussein | 6133