عنوان المقالة:RP TLC analysis of new antidepressants in pharmaceutical preparations RP TLC analysis of new antidepressants in pharmaceutical preparations
A simple and precise reversed-phase thin-layer chromatographic (RP TLC) method for simultaneous separation of fluoxetine and citalopram in pharmaceutical preparations has been developed and validated. Separation was performed on RP-18 F254 TLC plates with methanol-0.05 M phosphate buffer (pH 5)-triethylamine 68:27:5 (v/v) as mobile phase. Densitometric detection was performed at 230 nm. The method was validated for linearity, accuracy, precision, selectivity, and robustness. Calibration plots showed the response, as peak area, was a linear (r2 > 0.9988) function of the amounts of the compounds in the concentration range 500–5000 ng per spot. Statistical analysis proved the method was both precise and accurate. The method was successfully used for analysis of citalopram and fluoxetine in their pharmaceutical preparations, with recovery of the compounds ranging from 99.10 to 101.70% of the labeled amount.
إبراهيم علي أحمد عمار | Ibrahim Ali Ahmed Amar | 15358