عنوان المقالة:الكشف الجزيئي ومقاومة المضادات الميكروبية لبكتريا perfringens Clostridium المعزولة من مرضى السكري والجروح الناجمة عن الاطلاقات النارية Molecular detection and antimicrobial resistance of Clostridium perfringens isolated from diabetic patients and bullet wounds
د. ميساء صالح مهدي الشكري | Dr. Maysa Salih Mahdi Al-Shukri | 11155
نوع النشر
مجلة علمية
المؤلفون بالعربي
علياء محمد حمود، ميساء صالح الشكري، علاء هاني الجراخ
المؤلفون بالإنجليزي
Aliaa Mohamed Hmood, Maysa S M Al-Shukri, Alaa H Al-Charr
الملخص العربي
Clostridium perfringens is a major cause of gas gangrene. The morbidity of C. perfringens is connected with producing toxins. This cross-sectional study was designed to isolate, genetically diagnose, and study the antibiotic susceptibility patterns of C. perfringens isolated from clinical samples. Different wound swabs (from diabetic patients, cellulitis, and bullet wounds) were taken from 140 patients. For isolation of anaerobic bacteria, samples (in thioglycolate broth) were immediately incubated anaerobically then identified according to the cultural properties and biochemical tests. DNA was extracted from all specimens. Polymerase chain reaction was applied for detection of 16SrRNA and internal transcribed spacer (ITS) genes of C. perfringens. The susceptibility of bacterial isolates to different antibiotics was determined using Vitek 2 system and disk diffusion test. Out of 140 clinical samples collected during this study, 3 (2.14%) C. perfringens isolates were recovered of which 2 isolates (1.43%) obtained from diabetic patients and one (0.71%) from bullet wounds. Results also showed that only 7 isolates (5%) were detected by a molecular method using specific primers 16S rRNA and ITS genes of C. perfringens. Results of antibiotic susceptibility testing showed that all isolates were highly susceptible to penicillins and β-lactamase inhibitors, metronidazole, and aminoglycosides. On the other hand, all isolates were highly resistant to tetracycline, levofloxacin, and erythromycin. The susceptibility patterns of C. perfringens isolates showed that all isolates were multidrug resistance. Using the amplification of ITS gene increases specificity and sensitivity (by reducing non-specific annealing and primer dimer formation) which increases the probability of detection of suspected C. perfringens isolates.
تاريخ النشر
20/01/2019
الناشر
Journal of Applied Biology & Biotechnology
رقم المجلد
7
رقم العدد
1
رابط DOI
10.7324/JABB.2019.70110
الصفحات
54-59
رابط الملف
تحميل (352 مرات التحميل)
رابط خارجي
http://jabonline.in/admin/php/uploads/321_pdf.pdf
الكلمات المفتاحية
Molecular detection, Internal transcribed spacer gene, Antibiotic susceptibility, Clostridium perfringens, Diabetic patients
رجوع