Mohammad-BorhanAl-Zghoul, Zuhair BaniIsmail, Abd Elhafeed S.Dalab, Abdulla Al-Ramadan, Thnaian A. Althnaian, Saeed Y.Al-ramadan, Abdelhadi M. Ali, Ibrahim F.Albokhadaim, Khalid Ahmed Al Busadah, Abdulhakeem Eljarah, Khaleel I. Jawasreh, Kevin M.Hannone
الملخص الانجليزي
The effect of thermal manipulation (TM) during embryogenesis (ED 12–18) on mRNA expressions of heat shock proteins (Hsp90, Hsp60 and HSF-1) in muscle, heart and brain tissues during thermal challenge (TC) at post-hatching days 10 and 28 was investigated. Fertile chicken eggs were randomly divided into four groups: Control group (37.8 °C), TM1 (39 °C for 9 h), TM2 (39 °C for 12 h) and TM3 (39 °C for 18 h). At days 10 and 28 of age, chicks in TC groups were subjected to thermal challenge (TC) at 43.0 °C for 6 h while naïve chicks were kept under regular conditions. When compared with the control, TM resulted in a significant increase in mRNA levels of Hsp90, Hsp60 and HSF-1in muscle, heart and brain tissues during embryogenesis and during TC at days 10 and 28 post-hatching. These results indicate a long-term enhancement of Hsp90, Hsp60 and HSF-1 gene expressions associated with improved thermotolerance acquisition in thermally manipulated chicks.
تاريخ النشر
30/04/2015
الناشر
Research in Veterinary Science
رقم المجلد
رقم العدد
رابط DOI
https://doi.org/10.1016/j.rvsc.2014.12.014
الكلمات المفتاحية
Heat shock protein 90 (Hsp90)Heat shock protein 60 (Hsp60)Heat shock factor-1 (HSF-1)ThermotoleranceThermal manipulation
عبد الحفيظ سمير عبد الكريم دلعب | Abdelhafeed Sameer Abdulkarem Dalab | 2299