Abstract
The aim of study make Phylogenetic trees of mutant Staphylococcus aureus produce recombinant thrombolytic enzyme. Isolation S.aureus from different clinical sources from human that identified by vitek2-GP : 280 (56%) tonsils , 100 (20%) nose, 40 (8%) tumors, 17 (3.4%) urine, 27 (5.4%) skin(Acne) and 36 (7.2%) Blood. Genotypic analysis of sak gene showed presence of sak gene in all mutant VSSA and MSSA S.aureus after done chemical mutagenesis compared with control before chemical mutagenesis, size of sak gene (492bp) compared with DNA Ladder (1500bp).DNA Sequencing of S.aureus elucidate the coding region for Sak gene amplified from mutant VSSA and MSSA compared with analogous sequence of the same gene from standard strain of S.aureus possessed in BLAST / NCBI on the web site. Register strain in NCBI(National Center of Biotechnology Institute) Strain carry ID in NCBI/Gene Bank : strain1 ID=MN907801.1,strain2 ID=MN907802.1, strain3 ID=MN907803.1, strain4 ID=MN907804.1, strain5 ID=MN907805.1 and strain6 ID=MN907806.1; convert nucleotide sequence to protein by using Mega 6 program software. Phylogenetic trees drawn by neighbor-joining trees of strain based on spa-X Gene sequences for all strain that recording in NCBI/GeneBank hold accession number MN907801.1, MN907802.1, MN907803.1, MN907804.1, MN907805.1, MN9078026.1 Staphylococcus aureus IRAQ, the relativity between strain 0.9893-1.5814(99%); the relativity between 6 strain with strain in Australia 1.916(100%) 12/ CP043389.1:209416-2095276 Staphylococcus aureus Australia Meliboume; the relativity with strain 8 USA:KY America (100%), also the relativity with strain19 (100%) CP020960.1:153230:953990 Staphylococcus aureus Canada: Calgary (100%) relativity with strain(12,13,14,15,16,17,21,18), this results relativity with other strain in the world.The relativity of IRAQ strain between strain MN907801.1 with strain MN907804.1 0.8960(100%); the relativity between MN907804.1 with strain MN907805.1 is 0.9000(99%); the relativity between strain MN907801.1 and MN907805.1 is 0.3175(99%); the relativity of strain MN907801.1 with (relativity between MN907806.1, MN907805.1, MN907804.1, MN907801.1 is (99%)) 0.3038 (99%); the relativity between MN907803. (relativity between all strain) 0.2933(99%) and shown link in NCBI/GeneBank for strain recording with number 060753351. Compatibility IRAQ strain with other strain in the world is 99% in France, Japan, China(Zhejiang) province, USA (New York city),Ghana: Accra, Japan:Kyoto, Australia:Melbourne, USA:Detroit, USA:VA, China, USA:California, Canada:Calgary, USA, USA:SanDiego, Canada: Calgary.
نبراس رضا محمد | Nebras Rada Mohammed | 19388