عنوان المقالة:Molecular Copro-Prevalence of Cryptosporidium in Egyptian Children and Evaluation of Three Diagnostic Methods
أيمن البدرى | Ayman A. El-Badry | 6808
نوع النشر
مجلة علمية
المؤلفون بالعربي
Fathy, M.M., Abdelrazek, N.M., Hassan, F.A., El-Badry, A.A.
الملخص العربي
Objective To determine molecular prevalence of Cryptosporidium in a cohort of Egyptian children and compare three diagnostic tests. Methods Stool samples from children with diarrhea (n=150) and from apparently healthy children (n=100) were examined for Cryptosporidium using microscopy, enzyme linked immuosorbant assay (ELISA) and nested polymerase chain reaction (nPCR). Results nPCR detected Cryptosporidium in 22.4% of children. Acid-fast stain and ELISA showed false negativity but 100% specificity with nPCR as gold standard. Conclusion Cryptosporidium is a common cause of diarrhea in children in Egypt.
تاريخ النشر
09/01/2014
الناشر
Indian Pediatr.
رابط DOI
10.1007/s1
رابط خارجي
http://www.indianpediatrics.net/sep2014/727.pdf
الكلمات المفتاحية
DiarrheaEtiologyELISANested PCR
رجوع