عنوان المقالة:التأثير التازري لتقنيات ترشيح النطف ومضادات الأكسدة المضافة إلى السائل المنوي للجاموس العراقي SYNERGISTIC EFFECT OF SPERM FILTRATION TECHNIQUES AND ANTIOXIDANTS ADDED TO EXTENDER OF IRAQI BUFFALO SEMEN
This study investigated the synergistic effect of glass wool and sephadex sperm filtration techniques on
improving the low semen quality of buffalo bulls with or without antioxidants. Semen was collected for
14 weeks, evaluated weekly, and divided into 12 groups. Good and low semen have extended using a
Tris extender. Good semen quality is divided into three groups [S1: Tris extender; S2: Tris + vitamins
E (2 mM) and C (5 mM); S3: Tris + glutamine (20 mM) and arginine (1 mM)]. Low semen quality was
divided into three main groups and subdivided into three sub-groups (S4; Tris extender; S5: Tris
+vitamins E (2 mM) and C (5 mM); S6: Tris + glutamine (20 mM) and arginine (1 mM)). In the 2nd
and 3rd main groups, glass wool and sephadex techniques are used with or without adding vitamins
(E+C) and amino acids (Glutamine and arginine) and subdivided into three sub-groups with each
technique, referred to as S7–S9 for glass wool and S10–S12 for sephadex techniques. Improving
(P<0.01) motility, normal morphology, acrosome, and plasma membrane integrity and reducing total
sperm abnormalities of low semen quality were noticed using sephadex with or without adding
vitamins or amino acids post-cryopreservation. In conclusion, the sephadex technique removed
immotile sperm, dead, abnormal, and good sperm harvested by this technique, which in turn may
reflect positively on improving the pregnancy rate.
د. سيف ناظم الوائلي | Dr. Saif N. Alwaeli | 4715