The 14 bp insertion/deletion gene polymorphism of
insertion /deletion (+14 bp -14 bp) in the exon 8 of
(HLA-G) human leucocyte antigen-G gene has been
usually recognized to associate with frequent abortion
(RA). This study was designed to determine the possible
association between 14bp of HLA-G deletion/ insertion
gene polymorphism with frequent abortion in
husband's relatives and husbands strange. Peripheral
blood was collected from ninety (90) women with
periodic abortion, three abortions or more with
unknown causes (husband's relatives was 58 and
husband's strange was 32). DNA from the peripheral
blood was extracted, the concentration and purity of
DNA were measured then, DNA primer was designed
by using genious software program. HLA-G gene of the
both groups was amplified by using polymerase chain
reaction (PCR) and analyzed by gel electrophoresis.
The result revealed that homozygous insertion
(+14bp/+14bp) was in husband's relatives (13), and
husband's strange (11), and homozygous deletion (-
14bp/-14bp) results were in husband's relatives (11),
and husband's strange (5) while heterozygous (+14bp/-
14bp) results were in husband's relatives (34), and
husband's strange (16).
In the present study any significant variation was not
found between heterozygous and homozygous amongst
husband's relatives and husbands strange in Iraqi
women. We also did not found any implication between
husband's relatives and husbands strange in the field of
alleles (+14bp (insertion) or -14bp (deletion) in
recurrent abortion).
محمد جبير مهيدي | Mohammed Jobair Muhaidi | 3583