عنوان المقالة: Genetic Diversity of Fusarium solani f. sp. cucurbitae, the Causal Root and Crown Rot of Cucurbits (Melon) by Using Molecular Markers and Control
د حليمة زغير حسين | Halima Zugher Hussein | 6101
نوع النشر
مجلة علمية
المؤلفون بالعربي
المؤلفون بالإنجليزي
Falah Abdul-Hasan, Halima Z. Hussein
الملخص العربي
Detection of F. solani f. sp. cucurbitae causal agent of the crown and root rot disease of melon race 1, race 2 is difficult. It is based only on morphological characteristic. In this study, forty isolates identified as Fusarium solani based on morphological characterization, F. solani was one of the most frequently isolated species. Molecular identification of these isolates by PCR technique using species-specific primer, indicated that thirty-two isolates, amplified product 580 bp (race 1) and two isolate amplified product 580 bp (race 2), while six isolates were not amplified with primer of both races. Production of Trichothecenes (T2-toxen, DON.) by Fusarium solani was conducted on isolates confirmed as belonging in the F. solani by PCR. The results indicated that the presence of Tri5, Tri13 genes is coding the ability of synthesis mycotoxin. In vitro, the results indicated that NPs (AgNPs, MgNPs) and chemical (Phylex) possess the antifungal properties against at various level. Treatment with (AgNPs 150 ppm, MgNPs 2%, 3% ppm) and 3% Phylex resulted in maximum inha- bitation of F. solani. In vivo, five characters (height plant, hoot ant root fresh and dry
تاريخ النشر
11/04/2016
الناشر
American Journal of Plant Sciences, 2016, 7, 2151-2172
رقم المجلد
7
رقم العدد
2
ISSN/ISBN
2178
الصفحات
21
رابط الملف
تحميل (142 مرات التحميل)
الكلمات المفتاحية
Fusarium solani f. sp. cucurbitae Race 1, Race 2, Crown and Root Rot of Melon, PCR Detection, Specific Primers, Mycotoxins, Antifungal Effect, AgNPs, MgNPs, Phylex
رجوع