عنوان المقالة:Innovative artificial-intelligence-based approach for the biodegradation of feather keratin by Bacillus paramycoides, and cytotoxicity of the resulting amino acids. Innovative artificial-intelligence-based approach for the biodegradation of feather keratin by Bacillus paramycoides, and cytotoxicity of the resulting amino acids.
د زياد موسى عبد المعطي | Zeiad Moussa Abd El-Moati | 6770
نوع النشر
مقال علمي
المؤلفون بالعربي
المؤلفون بالإنجليزي
Moussa, Z., Darwish, D., Alrdahe, S., and Saber, W. I.
الملخص الانجليزي
The current study reported a new keratinolytic bacterium, which was characterized as Bacillus paramycoides and identified by 16S rRNA, and the sequence was then deposited in the GenBank (MW876249). The bacterium was able to degrade the insoluble chicken feather keratin (CFK) into amino acids (AA) through the keratinase system. The statistical optimization of the biodegradation process into AA was performed based on the Plackett–Burman design and rotatable central composite design (RCCD) on a simple solid-state fermentation medium. The optimum conditions were temperature, 37◦C, 0.547 mg KH2PO4, 1.438 mg NH4Cl, and 11.61 days of incubation. Innovatively, the degradation of the CFK process was modeled using the artificial neural network (ANN), which was better than RCCD in modeling the biodegradation process. Differentiation of the AA by high-performance liquid chromatography (HPLC) revealed the presence of 14 AA including essential and nonessential ones; proline and aspartic acids were the most dominant. The toxicity test of AA on the HepG2 cell line did not show any negative effect either on the cell line or on the morphological alteration. B. paramycoides ZW-5 is a new eco-friendly tool for CFK degradation that could be optimized by ANN. However, additional nutritional trials are encouraged on animal models.
تاريخ النشر
22/10/2021
الناشر
Frontiers in Microbiology
رقم المجلد
12
رقم العدد
رابط DOI
doi: 10.3389/fmicb.2021.731262
الصفحات
731262
رابط الملف
تحميل (0 مرات التحميل)
رابط خارجي
https://www.frontiersin.org/articles/10.3389/fmicb.2021.731262/full?utm_source=F-NTF&utm_medium=EMLX&utm_campaign=PRD_FEOPS_20170000_ARTICLE
الكلمات المفتاحية
keratinase, bioremediation, biodegradation, artificial neural network, mineral salts, artificial intelligence, response surface methodology
رجوع