Hydatid cysts were taken from 18 goats living in
different places in Iraq after diagnosing and specifying
the place of the injury for the period from July 2016 till
February 2018. DNA was extracted from
(protoscoleces) by using the mechanical crashing
method to gain an enough quantity of the DNA to
perform the partial diagnostic. NADH dehydrogenase
subunit 1 Gene (ND1) was used as a goal after
designing the specific primers for study.
Results showed that a DNA fragment was obtained
from all the samples that have been studied where the
primary detection of the gene depends on the molecular
size of about 800 bp after electrophoresis on Agaros
gel. After that, the results were refined by using
Geneaid Kit, Kuria and the purity of the DNA was
measured by using Spectrophotometer by measuring
the wave lengths 260 and 280 and then specifying the
value of (OD). After receiving the gene ND1 series,
they were matched with International Gene Bank NCBI
where all the symbols gave matching results with G1
sheep strain.
محمد جبير مهيدي | Mohammed Jobair Muhaidi | 3546