عنوان المقالة:تأثير تقانتي اختيار Sil-select وswim-down مع إضافة مضادات الأكسدة إلى المخفف على صفات السائل المنوي لثيران الجاموس The effect of Sil-select and swim-down techniques with antioxidant added to diluent on buffalo bull’s semen traits
A study investigated the effect of sil-select and swim-down sperm selection protocols
on enhancing the bad semen attributes of buffalo bulls with or without antioxidants. Semen was
evaluated weekly (14 weeks) and divided into 12 groups. Good (GSQ) and bad (BSQ) semen
were diluted using a Tris diluent. The GSQ was divided into three groups [CS1: Tris diluent;
CS5: Tris+ vitamins E (2 mmol) and C (5 mmol); CS6: Tris +glutamine (20mmol) and arginine
(1mmol)]. The BSQ was divided into three groups and three sub-groups (CS2: Tris diluent; CS7:
Tris +vitamins E and C; CS8: Tris+ glutamine and arginine). In the 3rd and 4th main groups, the
Sil-Select (CS3) and swim-down (CS4) techniques were used with or without adding
antioxidants and subdivided into three sub-groups, referred to as CS9-CS10 for the sil-select
method and CS11-CS12 for the swim-down. Improving normal morphology (NM), acrosome,
plasma membrane integrity and lesser sperm abnormalities (SAB) were noticed in fresh semen
of the CS3 than the CS2 groups. The CS3, CS4, and CS9-CS12 groups recorded higher NM and
lower SAB than other groups post-cryopreservation. The two techniques removed SAB and
harvested good sperm, which may improve the pregnancy rate and agricultural sustainability
systems.
د. سيف ناظم الوائلي | Dr. Saif N. Alwaeli | 4808