Ali H. Alwan, Ashraf S. Hassan, Noor N. Khwen, Haneen A. Kareem, Rana A. Sahib
الملخص العربي
ABSTRACT
Background/Purpose: This study aimed to prepare a new natural medium using Phoenix dactylifer to test the ability of low and moderate Density Biofilm Production of E.coli isolates on traditional media. Methods and Results: Escherichia coli is one of the most predominant pathogens, causing 80–90% of all episodes of UTIs and is a troublesome health problem in many different countries worldwide. Fifty isolates of Escherichia coli form patients with urinary tract infection in some Baghdad city hospitals were isolated. All isolates were test for their sensitivity to12 of common used antibiotics, the results showed that the isolates appeared sensitive, resistant or intermediate to antibiotics under study. Ability to produce biofilms of isolates was different that were test by using two methods (Congo-red agar methods-CRA- and Tissue culture plate methods-TCP-), the results showed that the number and percentage of isolates were 33(66%) positive, 12(26%) negative ,5(10%) non-identified by (CRA) while the( TCP) method showed that the 36(72%) strong,2(4%) weak and 12(24%) non-adherent. The isolates numbered (29) which was negative on (CRA) non-adherent on (TCP), (32) which was positive on (CRA) weak on (TCP) were selected to test the ability of new medium to stimulate formation of biofilm, in addition to isolate numbered (27) which was positive on (CRA) strong on (TCP) as a control. Our findings showed that the new medium stimulate the isolates 29 to become positive on (CRA) and strong on (TCP) ,while the isolate 32 became strong on(TCP). New medium preparation: This step was done by adding 2 ml (2.5mg/L) for each 20 ml of Congo-red- agar prepared already as , It was prepared by dissolving 37gm of Brain heart Infusion broth, 50gm Sucrose and 10gm Agar agar in 900 ml of distilled water then sterilized by autoclaving, cooling it, the stain was prepared by dissolving 0.8gm of stain in100 ml of distilled water and sterilized by autoclave then added to the above media after cooling it to 550C, then poured into sterile petri plates. This media used to detect on biofilm production. Plant extraction was poured into petri dish then the Congo-red- agar was added. Conclusion: Our study concludes reliable method to detect biofilm forming microorganisms. When compared to (Congo-red agar methods-CRA- and Tissue culture plate methods-TCP-) recommended as a general screening method for detection of biofilm producing bacteria in laboratories. The new medium could stimulate E .coli isolates to convert from low and moderate to high production of biofilm that gives confirmative way to diagnose some isolates with low or moderate biofilm production on traditional medium. Significance and Impact of Study : This study aimed to prepare a new natural medium using Phoenix dactylifer to test the ability of low and moderate Density Biofilm Production of E.coli isolates on traditional media. Some of these diagnostic methods are useful indicators for investigating infection but are nonspecific. Our results provided new method to diagnose some causative agents producing low or moderate biofilm by using a local new medium.
أ.م. اشرف سامي حسن | Assist prof.Ashraf S.Hassan | 7191