عنوان المقالة:تقنيات التحليل الحيوى والبايوكيميائية لعزل مشتقات الفانيلين لشجرة فاكهة البرقوق (1) Biotechnology and Biochemical Analysis Techniques to isolate Vanillin derivatives for Plum F ruit Tree (I)
انعام اسماعيل يوسف,علاء جعفر المحراث وطارق حسين مغير
المؤلفون بالإنجليزي
Mahrath, AJ; Yousif, EI; Al-Mgheer, TH
الملخص العربي
This application studied some biochemical and technological techniques to identify, isolate and purified some of biological molecules from fruits of Plum tree . The study involved drying the sample with freeze dryer for 24 hrs . Then samples were performed with Soxhlet apparatus by protic solvent (PSI), aprotic solvent (ASI), and aprotic –protic solvent interaction( PASI) were used to extracted the molecules . Then recovered the excess solvent vy evaporation and condensation. The sample directly transformed to the flash column chromatography technique (FCC)and monitored by Thin layer chromatography (TLC) to isolate m-methoy-p-hydroxy benzoic acid (vanillic acid) or (VA) and m-methoxy-p-hydroxy ethyl Benzoate (Ethyl Vanillate) or EV in about 95% pure state. These molecules AV and EV elucidated by spectroscopic tools (UV-Vis, ATR-IR, 1H-NMR, 13C-NMR, and Mass spectrometry).
ا.د. انعام اسماعيل يوسف | Enaam Ismail Yousif | 22477