Somia H. Abd allah, Sally M. Shalaby, Eman Abd-Elbary, Ayman A. Saleh, Mohammed Abu El-Magd
الملخص العربي
Background aims. Adult stem cellebased therapy is a promising novel approach for treatment of acute lung injury (ALI). In
this study, we evaluated the therapeutic effect of isolated human peripheral blood CD34þ progenitor cells in an ALI rat
model, induced by oleic acid (OA) injection. Methods. Seventy-five adult female rats were used in this study. Group A,
control without treatment, and group B, control injected with phosphate-buffered saline, comprised 15 rats each; the
remaining 45 rats were injected with OA to induce ALI and were further subdivided into 3 groups: group C (ALI group, 15
rats), group D (ALI and fibroblast group, 15 rats) and group E (ALI and CD34þ cell group, 15 rats). Results. CD34þ cells
transplantation in rats with OA-induced lung injury improves the arterial PaO2 and wet/dry ratio, reduces infiltration of
inflammatory cells and decreases lung vascular permeability as determined by reduced intra-alveolar and interstitial patchy
congestion and hemorrhage as well as decreased interstitial edema. Additionally, lung inflammation determined by
expression of the pro-inflammatory mediators intercellular adhesion molecule 1 and tumor necrosis factor-a was attenuated
in CD34þ celletreated rats at 6, 24 and 48 h post-OA challenge compared with non-treated rats. Moreover, the expression
of anti-inflammatory molecule interleukin-10 was up-regulated in the lung of OA-induced ALI rats after administration of
CD34þ cells. The important finding was that human TNF-a-induced protein 6 (TSG-6) gene expression was significantly
up-regulated in rats treated with CD34þ cells. Conclusions. The freshly isolated human peripheral bloodederived CD34þ
cells may be used as an important source of stem cells that improve ALI. The anti-inflammatory properties of CD34þ cells in
the lung are explained, at least in part, by activation of CD34þ cells to express TSG-6.
محمد رزق ابو المجد الغنام | Mohammed Rizk Abu El-Magd El-Ghannam | 8773