• Azme Dagga, Mohamed Abdel Aziz, Abed Al'raoof Al Amnama, Mervat Al-Sharif and Mahmoud El Hindi.
الملخص العربي
Gaza strip is a narrow piece of land lying in the coast of the Mediterranean Sea.
Its position on the cross roads from Africa to Asia made it a target for occupiers
and conquerors over the centuries. The Gaza Strip is situated in the south part
of historical Palestine and southeast of the mediterranean. Ten soil samples
were collected from different agricultural and non-agricultural soils from
different locations in Gaza strip, and all samples of Bacillus thuringiensis
strains were isolated from soil. B. thuringiensis is a gram positive and spore
forming bacterium. The most of the natural habitat for this bacterium is soil and
it is capable of producing the diversified varieties of crystal proteins (cry
proteins) with insecticide property. Polymerase Chain reaction (PCR) is a
molecular tool widely used to characterize the insecticidal bacterium B.
thuringiensis. This technique can be used to amplify specific DNA fragments
and thus to determine the presence or absence of a target gene. The molecular
identification of Bt cells through the PCR analysis of the delta-endotoxins
genes coupled to ribotyping, is an innovative method, that has enabled the
identification of this organism into wetland environments.
تاريخ النشر
10/04/2016
الناشر
International Journal of Current Microbiology and Applied Sciences
محمود وليد إبراهيم الهندي | MAHMOUD W I EL HINDI | 4652