Abstract. Hyperglycemia is considered a major initiator of oxidative stress which leads to
the formation of free radicals and consequently lipid peroxidation occurs, which leads to
tissue damage and diabetes mellitus development. Free radicals have been defined as
intermediates of some biological redox reactions necessary for the maintenance of life. In
presence of a free radical initiator and oxygen they may be oxidized this leading to lipid
peroxidation, as it was suggested, might be associated with running out of hydrogen. In
particular lipid peroxidation measured as levels of malondialdehyde (MDA). Glutathione
(GSH), an intracellular thiol causes the eradication of free radicals or reduction in hydrogen
peroxide level on state of oxidative stress. Decrease in the reduced GSH level has been
reported in the erythrocyte of diabetics. Decrease in the level of GSH occurs both due to the
competition between aldose reductase and glutathione reductase for NADPH, a cofactor, and
increased oxidative stress (increased ratio of NADH/NAD). This study was conducted on 60
subjects. Informed consent was obtained from each person who agreed to participate in this
study, while the survey was based on standardized interviews and questionnaire. Subjects
were grouped as type 2-diabetics and healthy control with fasting plasma glucose (FPG) <
5.50 mmol/L. The ages ranged from 18 to 50 years. Glucose was determined by an enzymatic
colorimetric test on basis of Trinder-Reaction. MDA was performed as described by Lipid
Peroxidation Assay Kit (Colorimetric/Fluorometric), while the levels of GSH in all subjects
were measured by the DetectX® Glutathione kit. The results show a significant elevation (P ≤
0.05) in levels of glucose and MDA of type 2- diabetic patients in comparison with healthy
subjects, which reached to 16.30 ± 0.50 mmol/L and 0.87 ± 0.19 nmol/mL for the type 2-
diabetic patients, and 4.60 ± 0.15mmol L and 0.29 ± 0.5 nmol/mL for the control group,
respectively. Also, it has been found a significant decrease (P ≤ 0.05) in GSH levels in type
2- diabetic patients, which reached to 3.43 ± 0.91 μM/mL in comparison with the control
group which reached to 6.13±0.21 μM/mL. In conclusion we can observe that the increase in
glucose levels leads to free radical formation by auto-oxidation and increase in lipid
peroxidation (MDA levels), and inadequate antioxidant defense can occur during DM. In
addition, GSH deficiency will make the present state worse by increasing the oxidative stress,
since GSH is an important antioxidant.
تاريخ النشر
07/04/2020
الناشر
IOP Conf. Series: Journal of Physics: Conf. Series, 2nd International Science Conference
علياء ماجد منادي | Alyaa Majid Munadi | 3774