Nanoparticles represent a promising technology to enhance the efficacy of bioactive materials and a large
number of studies showed the effectiveness of nanostructured materials against various arthropod
species of economic importance. In this work nanostructured alumina (NSA) was prepared using sol-gel
method and the effect of NSA was evaluated as seed protectant against the main seed-infesting insect
pests Oryzaephilus surinamensis (L.) (Coleoptera: Silvanidae) Stegobium paniceum (L.) (Coleoptera: Anobiidae),
and Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae). Besides, we tested the
effects of NSA on seed germination and plant growth and finally, we assessed the presence of NSA as a
contaminant in the leaves of bean plants germinated from NSA-treated seeds. The results showed significant
insecticidal activity of NSA against the three tested species. After sixteen days, the percentage of
insect mortality at the highest NSA concentration tested (400 mg kg1) was 100.00% for S. paniceum
followed by O. surinamensis (80.64%) and T. confusum (79.41%). Besides, in-vitro tests indicated that NSA
has no effects on seeds germination and on radicle and shoot elongation. No effects of NSA were also
observed in pot tests on the bean’s plants. No differences were recorded in the leaves area, stoma density
and roots length. On the contrary, the shoot of plants from NSA-treated beans was about 66% higher than
the one of the non-treated plants (shoot, 15.07 cm for the control and 22.76 cm for NSA-treated plants).
Finally, no contamination by alumina particles was found by EDX-system coupled with Scanning Electron
Microscopy (SEM) on the surface of the P. vulgaris leaves obtained from NSA-treated beans. Overall, the
results showed that NSA could be an effective protective agent for the control insect pests during the
seeds storage.
كمال بلهامل | Kamel Belhamel | 2102