عنوان المقالة:Anticancer Drug 5-Fluorouracil in Aqueous Solution by Differential Pulse Polarography: An Assessment of Optimum Conditions Anticancer Drug 5-Fluorouracil in Aqueous Solution by Differential Pulse Polarography: An Assessment of Optimum Conditions
رزاق عبدالزهرة إبراهيم حمود العامري | Razzaq Abd Al-Zahra Ibrahim Alamery | 11300
نوع النشر
مجلة علمية
المؤلفون بالعربي
د.رزاق عبد الزهرة إبراهيم ،د.فلاح شريف عبد سهيل، د.حسين كاظم الحكيم
المؤلفون بالإنجليزي
Razzaq Abd Al-Zahra Ibrahim, Falah Shareef Abed Suhail, Hussein Kadhem Al-Hakeim
الملخص العربي
The potassium phosphate buffer as a supporting electrolyte of anticancer drug (5-fluorouracil (5- FU)) was the best among solutions of sodium phosphate buffer and Britton Robinson buffer in differential pulse polarography (DPP) at pH = 7.0 and T = 37 °C. The changes of temperature did not effect on inactivity of the supporting electrolyte (potassium phosphate buffer at T = 10-50 °C), and pH of the solution did not exceed 2% of each 5 °C (at pH = 7.0, by modified thermostat cell). However, the frequency measurements showed clear effect of temperature on diffusion current (IP / μA) of the chemotherapy compound in the range of 20-50 °C and under primary conditions. Then, the polarography measurements of 5-FU drug (at 10 μM, pH = 7 and T = 37 °C) gradually led to the optimum conditions: deposition potential =–0.9 V; drop size = 9.0 mm3 ; deposition time = 15.0 s; equilibration time = 5.0 s; pulse amplitude = 100 mV; pulse time = 7.0 ms; voltage step = 6 mV; voltage step time = 0.3 s; and sweep rate = 20.0 mV/s. The thermal assessment of 5-FU drug (after achievement of the optimum conditions) in a new thermostat vessel at HMDE by DPP showed that the reaction of 5-FU molecules represented pseudo first order reaction, instead of first order); the secondary waves of 5-FU drug may be due to formation of molecular complexes in aqueous solution and the reduction of 5-FU molecules at mercury surface electrode appeared as physisorption, instead of chemisorption.
الملخص الانجليزي
The potassium phosphate buffer as a supporting electrolyte of anticancer drug (5-fluorouracil (5- FU)) was the best among solutions of sodium phosphate buffer and Britton Robinson buffer in differential pulse polarography (DPP) at pH = 7.0 and T = 37 °C. The changes of temperature did not effect on inactivity of the supporting electrolyte (potassium phosphate buffer at T = 10-50 °C), and pH of the solution did not exceed 2% of each 5 °C (at pH = 7.0, by modified thermostat cell). However, the frequency measurements showed clear effect of temperature on diffusion current (IP / μA) of the chemotherapy compound in the range of 20-50 °C and under primary conditions. Then, the polarography measurements of 5-FU drug (at 10 μM, pH = 7 and T = 37 °C) gradually led to the optimum conditions: deposition potential =–0.9 V; drop size = 9.0 mm3 ; deposition time = 15.0 s; equilibration time = 5.0 s; pulse amplitude = 100 mV; pulse time = 7.0 ms; voltage step = 6 mV; voltage step time = 0.3 s; and sweep rate = 20.0 mV/s. The thermal assessment of 5-FU drug (after achievement of the optimum conditions) in a new thermostat vessel at HMDE by DPP showed that the reaction of 5-FU molecules represented pseudo first order reaction, instead of first order); the secondary waves of 5-FU drug may be due to formation of molecular complexes in aqueous solution and the reduction of 5-FU molecules at mercury surface electrode appeared as physisorption, instead of chemisorption.
تاريخ النشر
27/07/2018
الناشر
Nano Biomed. Eng.
رقم المجلد
10
رقم العدد
2
رابط DOI
DOI: 10.5101/nbe.v10i2.p117-128
الصفحات
117-128
رابط الملف
تحميل (235 مرات التحميل)
رابط خارجي
http://www.nanobe.org
الكلمات المفتاحية
Optimum conditions; Drug 5-fluorouracil (5-FU); Differential pulse polarography (DPP); Behavior of 5-FU
رجوع