Back ground: The oral mucosa mainly exposed to injury by trauma or pathologic diseases, Leptin is a hormone known to has many physiological roles that effects the cell function and, acts as wound healing accelerator. The aim of the present study is to evaluate the effect of topical application of recombinant leptin on induced traumatic oral ulcer healing by mean of immunohistochemical localization of Decorin.
Materials and methods: Forty eight male Albino rats age between 2-3 months with body weight between (200.45-270.53g),were subjected for traumatic ulcer by surgical blade on the right side of the buccal mucosa by surgical blade (no.15) ,with diameter of (8 mm). The animals divided into two groups ;control group : the ulcer treated with sterilized distal water, the experimental group: the ulcer treated with 10μl of 1 μg/ml recommbenant leptin.The rats were sacrificed at 3,7,10 days. Immunohistochemical methods were used to detect the expression of decorin in both control and study groups .
Results: The present study showed that the recompenant leptin treatment increased expression of decorin in ulcer area from the 3rd day of ulceration by epithelial cell, endothelial cells, fbroblast cells , with highly significant differences in comparison with control group .
Conclusion : leptin accelerated the healing process in oral mucosa ulcer by increase the expression of decorin in early healing period than control.
م.د. صابرين سامي عبد | Lec. Dr. Sabreen Sami Abed | 3002