عنوان المقالة: Ultrasound-assistance one-pot synthesis of 1,2,4,5-Tetrasubstituted Imidazole derivatives and their in vitro anti-urolithiasis activities
This study has focused on the evaluation of in vitro anti-urolithiasis properties of the novel
prepared imidazole derivatives. A new series of seven imidazole derivatives (5a–5g) were prepared
using a simple, multi-component, one-pot method via a cyclocondensation reaction of 1,2-
diketones, primary aromatic amines, aryl aldehydes, and ammonium acetate in the presence of
sulfuric acid as a catalyst. The reactions in traditional heating conditions were compared with
ultrasound-irradiation reactions. As an effective technique for green synthetics, ultrasound synthesis
is being employed increasingly to accelerate organic reactions. In comparison to traditional
methods, ultrasonication has better yields, faster reaction times, higher selectivity, and fewer side
effects. The all-synthesized compounds were identified using melting point, elemental analysis (C.
H.N) and various spectral analyses such as FTIR, NMR and mass spectra. Some of the prepared
compounds 5a,5b,5f and fg were then tested for anti-urolithiasis inhibitory properties via
nucleation and aggregation assays. The inhibitory activity was measured using an Elisa microplate
reader at λ = 620 nm, the results of which showed that compounds 5a and 5g have good
inhibitory activity in the nucleation assay, while compound 5a has good inhibitory activity in the
aggregation assay compared with the cystone as a positive standard.
هيثم دلول حنون | Haitham Dalol Hanoon | 6429