عنوان المقالة:Genotyping of Giardia duodenalis isolates from dogs in Guangdong, China based on multi-locus sequence
محمد وليد السراقبي | Muhamd AlSarakibi | 5423
- نوع النشر
- مجلة علمية
- المؤلفون بالعربي
- Zheng G, Muhamd Alsarakibi
- الملخص العربي
- This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci: glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal DNA (18S rRNA) genes. For this work, a total of 216 dogs' fecal samples were collected in Guangdong, China. The phylogenetic trees were constructed with MEGA5.2 by using the neighbor-joining method. Results showed that 9.7% (21/216) samples were found to be positive; moreover, 10 samples were single infection (7 isolates assemblage A, 2 isolates assemblage C, and 1 isolate assemblage D) and 11 samples were mixed infections where assemblage A was predominant, which was potentially zoonotic. These findings showed that most of the dogs in Guangdong were infected or mixed-infected with assemblage A, and multi-locus sequence typing could be the best selection for the genotype analysis of dog-derived Giardia isolates.
- تاريخ النشر
- 26/06/2014
- الناشر
- Korean Journal of Parasitology
- رابط DOI
- 10.3347/k
- رابط الملف
- تحميل (155 مرات التحميل)
- رابط خارجي
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4096643/pdf/kjp-52-299.pdf
- الكلمات المفتاحية
- Giardia duodenalis, dog, multi-locus sequence typing