عنوان المقالة:Antioxidant-enzyme reaction to the oxidative stress due to alpha-cypermethrin, chlorpyriphos, and pirimicarb in tomato (Lycopersicon esculentum Mill.)
Tomato (Lycopersicon esculentum Mill.) becomes
one of the world’s foremost vegetables, and its world production
and consumption have increased fairly quickly. The capacity
to induce oxidative stress in tomato plant, exposed to
three xenobiotics such as alpha-cypermethrin, chlorpyriphos,
and pirimicarb, was investigated by the evaluation of lipid
peroxidation by measuring malondialdehyde (MDA) rate; also,
we studied the response of tomato to this stress by
assessing the response of superoxide dismutase (SOD), catalase
(CAT), peroxidase (POD), ascorbate peroxidase (APX),
glutathione-s-transferase (GST), and glutathione reductase
(GR). The effect of the insecticides was observed using four
concentrations (25, 50, 75, and 100 %) for germinating seeds
and only the recommended concentration in agriculture
(100 %) for growing plants. Our results show an important
accumulation of MDA, demonstrating the increase of lipid
peroxidation in consequence of the excessive reactive oxygen
species (ROS) production due to insecticide treatment. In response
to this oxidative stress in tomato seedlings and plants,
the activities of antioxidant-enzyme system were generally
enhanced. The electrophoretic analysis showed also the apparition
of new isoenzymes as the case for CAT and POD.
عبد السلام النبيلي | Abdeslam Ennabili | 5771