عنوان المقالة: Human peripheral blood CD34+ cells attenuate oleic acid-induced acute lung injury in rats
محمد رزق ابو المجد الغنام | Mohammed Rizk Abu El-Magd El-Ghannam | 8903
- نوع النشر
- مجلة علمية
- المؤلفون بالعربي
- Somia H. Abd allah, Sally M. Shalaby, Eman Abd-Elbary, Ayman A. Saleh, Mohammed Abu El-Magd
- الملخص العربي
- Background aims. Adult stem cellebased therapy is a promising novel approach for treatment of acute lung injury (ALI). In this study, we evaluated the therapeutic effect of isolated human peripheral blood CD34þ progenitor cells in an ALI rat model, induced by oleic acid (OA) injection. Methods. Seventy-five adult female rats were used in this study. Group A, control without treatment, and group B, control injected with phosphate-buffered saline, comprised 15 rats each; the remaining 45 rats were injected with OA to induce ALI and were further subdivided into 3 groups: group C (ALI group, 15 rats), group D (ALI and fibroblast group, 15 rats) and group E (ALI and CD34þ cell group, 15 rats). Results. CD34þ cells transplantation in rats with OA-induced lung injury improves the arterial PaO2 and wet/dry ratio, reduces infiltration of inflammatory cells and decreases lung vascular permeability as determined by reduced intra-alveolar and interstitial patchy congestion and hemorrhage as well as decreased interstitial edema. Additionally, lung inflammation determined by expression of the pro-inflammatory mediators intercellular adhesion molecule 1 and tumor necrosis factor-a was attenuated in CD34þ celletreated rats at 6, 24 and 48 h post-OA challenge compared with non-treated rats. Moreover, the expression of anti-inflammatory molecule interleukin-10 was up-regulated in the lung of OA-induced ALI rats after administration of CD34þ cells. The important finding was that human TNF-a-induced protein 6 (TSG-6) gene expression was significantly up-regulated in rats treated with CD34þ cells. Conclusions. The freshly isolated human peripheral bloodederived CD34þ cells may be used as an important source of stem cells that improve ALI. The anti-inflammatory properties of CD34þ cells in the lung are explained, at least in part, by activation of CD34þ cells to express TSG-6.
- تاريخ النشر
- 04/08/2015
- الناشر
- Cytotherapy
- رابط الملف
- تحميل (250 مرات التحميل)
- الكلمات المفتاحية
- acute lung injury, CD34þve, ICAM-1, IL-10, TNF-a, oleic acid, TSG-6