عنوان المقالة:In Vitro Anti-Metastatic and Antioxidant Activity of Nicotiana glauca Fraction Against Breast Cancer Cells
سعد صبار دحام | Saad Sabbar Dahham | 8449
نوع النشر
مجلة علمية
المؤلفون بالعربي
Yasser M Tabana, Saad S Dahham, Loiy E Ahmed Hassan, Majed Ahmed Al-Mansoub, Mohamad Taleb-Agha,
الملخص العربي
Brest cancer is the most predominant cancer among the women. Drug discovery research and novel approaches to treat breast cancer has extensively been carried out worldwide. It goes without saying that herbal and traditional medicines play a significant role in the communities worldwide as it is considered as a source of effective remedies, especially against cancer. Nicotiana glauca (Solanaceae), or tree tobacco, is found in dry arid climates of North America, Africa, Europe and Australia. It is considered as a toxic plant in some places. However, N. glaucawas reported to be anodyne and virucidal. It is a folk remedy for boils, headaches, piles, sores, wounds and cancer. In the present study, we investigated the cytotoxic, Anti-metastatic and antioxidant activities as well as quantifying the major chemical constituents of the active fraction of N. glauca. The dichloromethane fraction of N-hexane extract displayed selective cytotoxicity against the MCF-7 cells with (IC 17.98 µg/mL). However, the extract did not show significant cytotoxicity against MDA (IC 85.94 µg/ml) 50 50 and normal cells (IC 80 µg/mL). The apoptotic effects were investigated on mitochondrial membrane potential, 50 cytoplasmic shrinkage and chromatin condensation. A mitochondrial apoptosis pathway was found to be involved in the cell death induced by the n-hexane fraction of N. glauca. Furthermore, the fraction demonstrated significant inhibitory effect on migration and invasion on MCF-7 cells. In addition, the fraction of N. glaucahas shown high antioxidant potential. The results indicate that N. glaucapossesses potent antitumor and antioxidant activities. Warrant further investigation is worthy to illustrate the potential chemotherapeutic agent.
تاريخ النشر
10/05/2015
الناشر
Advances in Biological Research
رابط DOI
DOI: 10.58
رابط خارجي
https://www.researchgate.net/profile/Majed_Al-Mansoub/publication/281346046_In_Vitro_Anti-Metastatic
الكلمات المفتاحية
MCF-7 (human hormone sensitive breast cancer cell line) MDA-MB-231 (Human hormone resistant breast
رجوع