عنوان المقالة:التقدير الطيفي لديكلوفيناك الصوديوم بأستعمال 4،2 -ثنائي نايترو فنيل ھيدرازين بشكله النقي وبمستحضراته الصيدلانية Spectrophotometric Determination of Diclofenac sodium Using 2,4-dinitrophenylhydrazine in Pure Form and Pharmaceutical Preparations
رؤى مؤيد محمود | Ruaa Muayad Mahmood | 9885
- نوع النشر
- مجلة علمية
- المؤلفون بالعربي
- سرمد بھجت ديكران - رؤى مؤيد محمود
- المؤلفون بالإنجليزي
- Sarmad B. Dikran, Ruaa M. Mahmood
- الملخص العربي
- A simple and sensitive spectrophotometric method is described for the determination of diclofenac sodium (DCL), in pure form and pharmaceutical formulations. The method is based on the oxidation of 2,4-dinitrophenylhydrazine (2,4-DNPH) and coupling of the oxidized product with DCL in alkaline medium to give intensively colored chromogen which exhibits maximum absorption (λmax) at 600 nm, and the concentration of DCL was determined spectrophotometrically. The optimum reaction conditions and other analytical parameters were evaluated. In addition to classical univariate optimization, modified simplex method (MSM) has been applied in optimization of the variables affecting the color producing reaction. Beer’s law is obeyed in the concentration range of 0.5-40 μg.mL-1 with molar absorptivity of 2.0994×104 L.mol-1.cm-1. The limit of detection was found to be 0.020 μg.mL-1 and the Sandellʼs sensitivity value was 0.0152 μg.cm-2. The proposed method was successfully applied to the determination of DCL in pharmaceutical preparations.
- تاريخ النشر
- 23/06/2015
- الناشر
- Ibn Al-Haitham Jour. for Pure & Appl. Sci.
- رقم المجلد
- 28
- رقم العدد
- 3
- الكلمات المفتاحية
- Spectrophotometric determination, Diclofenac sodium, 2,4- dinitrophenylhydrazine.