Mohammed Abu El-Magd, Abeer Zaki, Khalid Kahilo, Mohammed Barakat, Ibrahim Hassan,
الملخص العربي
Prolactin (PRL) plays a crucial role in the initiation and maintenance of lactation in animals. It is a potential
quantitative trait locus and genetic marker of production traits in dairy cattle. However, no SNPs was detected in,
and no association studies was conducted on, PRL gene in Egyptian buffalo. Therefore, this study aims to detect
PRL gene polymorphisms and to study their effect on Egyptian water buffalo. A PCR product of 245 bp, including
part of the promoter of PRL was amplified using PCR and subsequently, SSCP and nucleotide sequencing were
conducted to identify its different allelic patterns in Egyptian buffaloes. Our results revealed presence of a novel
T72C SNP in the PRL promoter with two genotypes (TC, CC). Chi-square test indicated presence of genetic
disequilibrium with respect to studied variations and revealed significant difference in distribution of variants among
buffaloes. Genetic diversity parameter showed moderate degree of polymorphism. The two genotypes (TC, CC) did
not show association with milk traits (milk yield, fat%, protein%, and lactose%, solid %) in Egyptian buffaloes. We
conclude that no significant difference in milk traits between Egyptian buffaloes have TC or CC genotypes and so
breeders should not depend on this SNP (alone) in their selection for dairy animals.
محمد رزق ابو المجد الغنام | Mohammed Rizk Abu El-Magd El-Ghannam | 9149