احمد مهدي سعيد و محمد جاسم حمزه و نور جاسم محمد علي
المؤلفون بالإنجليزي
Ahmed Mahdi Saeed , Mohammed Jassim Hamzah and Noor Jassim Mohammed Ali
الملخص العربي
تم تقدير الفيتامينات مضادات الاكسدة باستحدم طريقة طيفية
الملخص الانجليزي
A sensitive, simple, accurate and fast method for vitamin C and E determination in pure and drug formulations using spectrophotometric was developed. The developed method is based on the formation of the charge transfer complex via the reaction between vitamins and Fe+3 [FeNH4(SO4)2.12H2O] in the presence of K3Fe(CN)6 which lead the formation of a blue-greenish colored product that has a maximum absorption at λmax=743 nm. The optimum reaction conditions such as temperature, volume, reaction time and pH were studied. The linear dynamic range for the intensity versus vitamins concentrations are 0.05-28 and 0.5-28 μg/mL for vitamin C and E respectively, with LOD values of 0.01 and 0.09 μg/mL and LOQ values of 0.033 and 0.297 μg/mL. The correlation coefficient (R2) is 0.9993, while the percentage linearity (%R2) was 99.93%. %R.S.D for the repeatability (n=3) is < 0.3%. The method was applied successfully for the determination of vitamin C and E in pharmaceutical preparation. The new method can be accepted as an alternative analytical method for the determination of the mention vitamins in pure and dosage forms.
تاريخ النشر
01/08/2018
الناشر
International Journal of Pharmaceutical Sciences and Research
احمد مهدي سعيد محمد | Ahmed Mahdi Saeed Mohammed | 8750