Reversed-phase high performance liquid chromatography (RP-HPLC) with UV detection and gas chromatography with flame
ionization detection methods are described for the simultaneous determination of metronidazole and miconazole in vaginal ovules.
In the former, a C18 column was used with a mobile phase of methanol–water (40:60 v/v) at a flow rate of 1.0 ml min-1 with
detection at 220 nm, and sulfamethoxazole (SMX) used as an internal standard. Linear responses in the concentration ranges 10 -
/80 and 5 -/40 μg ml-1 with detection limits 0.93 and 0.45 μg ml-1 were found for metronidazole (MNZ) and micanozole nitrate (MIC)
respectively. Gas chromatography was performed on a capillary column (15 m × 0.32 mm i.d.) and nitrogen was used as a carrier gas
at a flow rate of 1.5 ml min-1. Linearity ranges were 200 - 1500 and 100 - 750 μg ml-1 for MNZ and MIC, with limit of detection values
of 3 and 3.6 μg ml-1, respectively, These method was found to be convenient and reproducible for the analysis of both active
ingredients in ovule dosage forms, and could be extended to the determination of other imidazoles (ketoconazale, econazole and
clotrimazole).
حسن سعد الديوه جي | Hasan Aldewachi | 5579