Yasser M Tabana, Saad S Dahham, Loiy E Ahmed Hassan, Majed Ahmed Al-Mansoub, Mohamad Taleb-Agha,
الملخص العربي
Brest cancer is the most predominant cancer among the women. Drug discovery research and novel
approaches to treat breast cancer has extensively been carried out worldwide. It goes without saying that herbal
and traditional medicines play a significant role in the communities worldwide as it is considered as a source
of effective remedies, especially against cancer. Nicotiana glauca (Solanaceae), or tree tobacco, is found in
dry arid climates of North America, Africa, Europe and Australia. It is considered as a toxic plant in some places.
However, N. glaucawas reported to be anodyne and virucidal. It is a folk remedy for boils, headaches,
piles, sores, wounds and cancer. In the present study, we investigated the cytotoxic, Anti-metastatic and
antioxidant activities as well as quantifying the major chemical constituents of the active fraction of N. glauca.
The dichloromethane fraction of N-hexane extract displayed selective cytotoxicity against the MCF-7 cells with
(IC 17.98 µg/mL). However, the extract did not show significant cytotoxicity against MDA (IC 85.94 µg/ml) 50 50
and normal cells (IC 80 µg/mL). The apoptotic effects were investigated on mitochondrial membrane potential, 50
cytoplasmic shrinkage and chromatin condensation. A mitochondrial apoptosis pathway was found to be
involved in the cell death induced by the n-hexane fraction of N. glauca. Furthermore, the fraction
demonstrated significant inhibitory effect on migration and invasion on MCF-7 cells. In addition, the fraction
of N. glaucahas shown high antioxidant potential. The results indicate that N. glaucapossesses potent
antitumor and antioxidant activities. Warrant further investigation is worthy to illustrate the potential
chemotherapeutic agent.
سعد صبار دحام | Saad Sabbar Dahham | 8429